Effects of androstenedione, insulin and luteinizing hormone on steroidogenesis in human granulosa luteal cells
نویسندگان
چکیده
منابع مشابه
Luteal macrophage conditioned medium affects steroidogenesis in porcine granulosa cells.
The purpose of the study was to examine the effect of luteal macrophage conditioned medium (LMCM) on progesterone and estradiol production by cultured granulosa cells. Porcine granulosa cells were cultured for 48 h with or without LMCM in the absence or presence of 100 ng/ml LH, FSH or prolactin. Progesterone and estradiol concentrations were measured by radioimmunoassay. Granulosa cells were a...
متن کاملThe somatostatin analogue, octreotide, modifies both steroidogenesis and IGFBP-1 secretion in human luteinizing granulosa cells.
The effect of the somatostatin analogue octreotide on the secretion of progesterone and insulin-like growth factor binding protein-1 (IGFBP-1) from human granulosa-luteal cells was investigated. Octreotide (10(-11), 10(-10) and 10(-9) M) alone induced a significant decrease in progesterone secretion (maximum suppression 69 +/- 5% of control: P < 0.0001). In contrast, treatment with octreotide i...
متن کاملPurine amplification of luteinizing hormone action in ovarian luteal cells.
Adenosine produced a severalfold amplification of luteinizing hormone (LH)-stimulated cAMP accumulation and progesterone secretion in rat luteal cells, but showed no similar effect on LH-stimulated cAMP accumulation and androgen secretion in Leydig cells. In the absence of LH, adenosine produced a small but significant stimulation of cAMP accumulation and steroid secretion in both luteal and Le...
متن کاملThe Effects of Lithium Chloride on Survival and Function of in Vitro Cultured Human Granulosa Cells
Purpose: To investigate the effect of lithium chloride (LiCl) on survival and functional activity of in vitro cultured human granulosa cells (GCs). Materials and Methods: GCs were isolated from follicular fluid of women undergoing IVF/ICSI. After RBCs removal, GCs were plated in a medium containing varying concentrations of LiCl including 1, 2 and 5mM for two weeks period. A culture without Li...
متن کاملEffects of fenvalerate on steroidogenesis in cultured rat granulosa cells.
OBJECTIVE This study was designed to examine the in vitro effects of fenvalerate on steroid production and steroidogenic enzymes mRNA expression level in rat granulosa cells. METHODS Using primary cultured rat granulosa cells (rGCs) as model, fenvalerate of various concentrations (0, 1, 5, 25, 125, 625 micromol/L) was added to the medium for 24 h. In some cases, optimal concentrations of 22(R...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Human Reproduction
سال: 2001
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/16.10.2061